Peptide microarrays coupled to machine learning reveal individual epitopes from human antibody responses with neutralizing capabilities against SARS-CoV-2기계 학습과 결합된 펩티드 마이크로어레이는 SARS-CoV-2에 대한 중화 기능을 가진 인간 항체 반응의 개별 에피토프를 나타냅니다.Article Published on 2022-12-012022-09-11 Journal: Emerging Microbes & Infections [Category] COVID19(2023년), SARS, 진단, 치료기술, 치료법, [키워드] anti-SARS-CoV-2 antibody Antibody binding assays binding epitope capture causative agent cellular coronavirus coronavirus SARS-CoV-2 Course COVID-19 detectable epitope Epitopes human antibody response IgA IgG IgM antibody immunoassays incubated infected with SARS-CoV-2 machine learning Microarray N-terminus negative control samples neutralization neutralization assays Neutralizing Neutralizing antibodies nine overlapping Patient peptide peptide arrays peptides positive protective effect Protective effects random Random forest response S protein SARS CoV SARS CoV-2 SARS-CoV-2 sera serology Serology. Specific structural protein structural proteins the disease the S protein vaccine candidate vaccine candidates Viral [DOI] 10.1080/22221751.2022.2057874 PMC 바로가기 [Article Type] Article
In silico characterization of mutations circulating in SARS-CoV-2 structural proteinsArticle Published on 2022-11-012022-11-16 Journal: Journal of biomolecular structure & dynamics [Category] SARS, 변종, [키워드] Algorithm Analysis caused circulating computational approach computational tool COVID-19 D614G death Deleterious discrete molecular dynamics DMD envelope protein full-length identify infections meta-data molecular molecular simulations mutant Mutation mutations N protein Neutral NMA non-structural protein normal mode analysis Nsp3 nucleocapsid nucleocapsid protein pandemic predicted Protein protein coding gene protein stability Proteins RdRP RNA-directed RNA polymerase SARS-CoV-2 SARS-CoV-2 genome SARS-CoV-2 genomes sequence Seven Spike protein stability structural protein suggested utilised viral dynamics viral genome viral pathogenicity Viral pathogenicity. [DOI] 10.1080/07391102.2021.1908170 PMC 바로가기
Cellular interferon-gamma and interleukin-2 responses to SARS-CoV-2 structural proteins are broader and higher in those vaccinated after SARS-CoV-2 infection compared to vaccinees without prior SARS-CoV-2 infectionArticle Published on 2022-10-172022-11-15 Journal: PLoS ONE [Category] COVID19(2023년), SARS, 진단, [키워드] assays BNT162b2 BNT162b2 vaccine class correlated demonstrated develop dose doses epitope exhibited Frequency greater healthcare worker healthy IFN-γ IgG response IL-2 IL2 Immunity individual infected with SARS-CoV-2 Infection Interferon-gamma interleukin-2 magnitude Moderna mRNA-1273 vaccine mononuclear cell mRNA-1273 N and M nine participant Participants PCR testing peptide Peripheral blood Pfizer-BioNTech positive Post-infection Proteins proteome repeated responders response responses against robust S glycoprotein S1 and S2 S2 subunit SARS-CoV-2 SARS-COV-2 infection structural protein subunit subunits T cell response T-cell Response the SARS-CoV-2 uninfected vaccination vaccinee was determined were expressed with COVID-19 [DOI] 10.1371/journal.pone.0276241 PMC 바로가기
Integration of Power-Free and Self-Contained Microfluidic Chip with Fiber Optic Particle Plasmon Resonance Aptasensor for Rapid Detection of SARS-CoV-2 Nucleocapsid ProteinArticle Published on 2022-09-232022-11-15 Journal: Biosensors [Category] SARS, 변종, 치료기술, 치료제, [키워드] antigen detection aptamer binding binding kinetics COVID-19 detect detection diagnostic fiber fiber optic biosensor global pandemic Gold nanoparticle infectious viruses integration leaking limit of detection localized surface plasmon resonance marker Microfluidic chip mutants N-protein novel coronavirus SARS-CoV-2 nucleocapsid nucleocapsid protein Optic Particle Plasmon POCT point-of-care Point-of-care testing. Rapid resonance risk robust SARS-CoV-2 single-stranded DNA ssDNA structural protein the SARS-CoV-2 the WHO virus [DOI] 10.3390/bios12100785 PMC 바로가기
Biophysical Modeling of SARS-CoV-2 Assembly: Genome Condensation and BuddingArticle Published on 2022-09-202022-11-16 Journal: Viruses [Category] SARS, 유전자 메커니즘, [키워드] assembly budding caused cellular condensation coronavirus 2 COVID-19 pandemic curtail de novo develop drug target envelope Genome genome condensation genomic RNA host cell identify Infectious virus Interaction intrinsic M protein mechanism membranes modeling molecular Mutation N protein oligomerization pathogen Protein protein intrinsic curvature. Research respiratory responsible resulting RNA SARS-CoV-2 spike structural protein structural proteins the S protein therapeutic target therapy Vaccines viral entry viral life cycle viral particle virus while [DOI] 10.3390/v14102089 PMC 바로가기
Metabolic dyshomeostasis induced by SARS-CoV-2 structural proteins reveals immunological insights into viral olfactory interactionsArticle Published on 2022-09-082022-11-16 Journal: Frontiers in Immunology [Category] SARS, 유전자 메커니즘, 치료기술, [키워드] accompanied Activation animal model bioinformatics cells characterized chemokine COVID-19 COVID-19 patients E protein expression Extracellular matrix immune immune response immunological immunomodulatory property Inflammation Interaction intrinsic kinases lipid metabolism Loss of Smell machine metabolic molecular multifunctional olfactory Protein proteome proteomic proteomics receptor reveal SARS-CoV-2 secretion Signaling Smell structural protein survival Symptom TGF-beta signaling the SARS-CoV-2 These data trajectory transcriptomics transcriptomics. tyrosine virion widespread [DOI] 10.3389/fimmu.2022.866564 PMC 바로가기
Evaluating the immunogenicity of gold nanoparticles conjugated RBD with Freund’s adjuvant as a potential vaccine against SARS-CoV-2Article Published on 2022-09-012022-11-15 Journal: Microbial pathogenesis [Category] COVID19(2023년), SARS, 진단, [키워드] adjuvant Antigen assays BALB/c mice boost candidate candidate vaccine cell-mediated immune response cell-mediated response cloned COVID-19 COVID-19 pandemic COVID-19 vaccine cytokine E. coli ENhance evaluated Evaluating examined expressed Freund's adjuvant global pandemic gold Gold nanoparticle Gold nanoparticles Health Host humoral Humoral immunity Humoral response IFN-γ IgG IgM IgM and IgG immune immune response immune system Immunogenic proteins Incomplete injected interval long-term immune response marker material Nano-vaccines outcome pandemic purified RBD RBD antigen RBD protein repeated SARS-CoV-2 SARS-CoV-2. significantly higher stimulate structural protein the RBD protein treatment group Vaccine while [DOI] 10.1016/j.micpath.2022.105687 PMC 바로가기
Structure of SARS-CoV-2 membrane protein essential for virus assembly바이러스 조립에 필수적인 SARS-CoV-2 막 단백질의 구조Article Published on 2022-08-052022-09-11 Journal: Nature Communications [Category] SARS, 변종, 치료법, [키워드] analysis component composed conformational changes conformations conserved coronavirus cryo Cryo-electron microscopy domain domains electron microscopy form hinge region Interaction ion channel M protein mechanism membrane protein morphogenesis nucleocapsid protein oligomers ORF3a Protein recruitment RNA SARS-CoV-2 SARS-CoV-2 ORF3a structural protein Structure the SARS-CoV-2 therapeutic intervention transmembrane virus [DOI] 10.1038/s41467-022-32019-3 PMC 바로가기 [Article Type] Article
Omicron mutations enhance infectivity and reduce antibody neutralization of SARS-CoV-2 virus-like particlesOmicron 돌연변이는 SARS-CoV-2 바이러스 유사 입자의 감염성을 높이고 항체 중화를 감소시킵니다.Article Published on 2022-08-022022-09-11 Journal: Proceedings of the National Academy of Sciences of [Category] COVID19(2023년), MERS, SARS, 변종, 진단, 치료기술, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 addition Antibody neutralization Antisera assembly B.1 B.1 lineage B.1.1 bebtelovimab Casirivimab Cell cell entry change convalescent sera coronavirus COVID-19 Delta detectable detrimental dose Effect Efficacy Efficiency eight eight subject elicited ENhance examined Imdevimab individual Johnson & Johnson Johnson & Johnson luciferase Moderna monoclonal antibodies monoclonal antibody Mutation N proteins neutralization neutralization activity neutralization titer Neutralizing antibodies neutralizing antibody omicron Omicron variant Patient Pfizer Pfizer/BioNTech Prevent property reduce reduced respiratory resulting robust SARS-CoV-2 SARS-CoV-2 variant sequence sera severe acute respiratory syndrome Coronavirus Spike protein Spread structural protein structural proteins subjects survivor Vaccine Vaccines variant variants Viral viral infectivity Virus-like particle virus-like particles virus-like particles. VLP VLPs [DOI] 10.1073/pnas.2200592119 PMC 바로가기 [Article Type] Article
SARS-CoV-2 nucleocapsid protein triggers hyperinflammation via protein-protein interaction-mediated intracellular Cl – accumulation in respiratory epitheliumSARS-CoV-2 뉴클레오캡시드 단백질은 단백질-단백질 상호작용 매개 세포내 Cl-호흡 상피에서의 축적을 통해 과염증을 유발합니다Article Published on 2022-07-272022-09-11 Journal: Signal Transduction and Targeted Therapy [Category] COVID19(2023년), SARS, 신약개발, 치료법, [키워드] abrogated Airway inflammation CAMP cell lines cells CFTR Concentration COVID-19 cystic fibrosis Cytokine storms Defense demonstrated depleting downregulated elicit epithelial epithelial cell epithelial cells expression fibrosis glucocorticoid Host host defense human airway epithelial hyperinflammation immune response immune responses Inflammatory response Inflammatory responses inhibition inhibitor Intracellular knockout microRNA modulating murine model murine models N protein pathogen phosphodiesterase Phosphorylation promoted Protein pulmonary inflammation raised reducing regulated respiratory epithelium resulting robust Rolipram SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 nucleocapsid second messenger selective serum SGK1 signaling pathway Smad3 structural protein suggested sustained targeting therapeutic strategy transmembrane Trigger upregulation [DOI] 10.1038/s41392-022-01048-1 PMC 바로가기 [Article Type] Article